Retroviral Vector Expression in TCR Transgenic CD4+ T Cells
YS Choi, S Crotty - T follicular Helper Cells: Methods and Protocols, 2015 - Springer
YS Choi, S Crotty
T follicular Helper Cells: Methods and Protocols, 2015•SpringerThe regulation of gene expression is key to understand the function of genes of interest. To
explore the biological functions of genes, transgenic knock-in or knockout technologies have
served as invaluable tools. While recent advances in molecular biology have introduced
new techniques (ie, CRISPR mediated gene editing)(Cong et al., Science 339 (6121): 819–
823, 2013; Wang et al., Cell 153 (4): 910–918, 2013) for the generation of transgenic mice in
a relatively short period of time, it can still take a long time to test biological hypotheses from …
explore the biological functions of genes, transgenic knock-in or knockout technologies have
served as invaluable tools. While recent advances in molecular biology have introduced
new techniques (ie, CRISPR mediated gene editing)(Cong et al., Science 339 (6121): 819–
823, 2013; Wang et al., Cell 153 (4): 910–918, 2013) for the generation of transgenic mice in
a relatively short period of time, it can still take a long time to test biological hypotheses from …
Abstract
The regulation of gene expression is key to understand the function of genes of interest. To explore the biological functions of genes, transgenic knock-in or knockout technologies have served as invaluable tools. While recent advances in molecular biology have introduced new techniques (i.e., CRISPR mediated gene editing) (Cong et al., Science 339(6121):819–823, 2013; Wang et al., Cell 153(4):910–918, 2013) for the generation of transgenic mice in a relatively short period of time, it can still take a long time to test biological hypotheses from scratch to design how to generate knock-in or knockout mice. Here, we describe methods to manipulate gene expression in T cell receptor (TCR) transgenic CD4 T cells, which allow us to investigate gene functions in the study of differentiation pathways of follicular helper T (Tfh) cells.
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