Impaired phagocytic function in CX3CR1+ tissue‐resident skeletal muscle macrophages prevents muscle recovery after influenza A virus‐induced pneumonia in old …

CE Runyan, LC Welch, E Lecuona, M Shigemura… - Aging cell, 2020 - Wiley Online Library
CE Runyan, LC Welch, E Lecuona, M Shigemura, L Amarelle, H Abdala‐Valencia, N Joshi
Aging cell, 2020Wiley Online Library
Skeletal muscle dysfunction in survivors of pneumonia disproportionately affects older
individuals in whom it causes substantial morbidity. We found that skeletal muscle recovery
was impaired in old compared with young mice after influenza A virus‐induced pneumonia.
In young mice, recovery of muscle loss was associated with expansion of tissue‐resident
skeletal muscle macrophages and downregulation of MHC II expression, followed by a
proliferation of muscle satellite cells. These findings were absent in old mice and in mice …
Abstract
Skeletal muscle dysfunction in survivors of pneumonia disproportionately affects older individuals in whom it causes substantial morbidity. We found that skeletal muscle recovery was impaired in old compared with young mice after influenza A virus‐induced pneumonia. In young mice, recovery of muscle loss was associated with expansion of tissue‐resident skeletal muscle macrophages and downregulation of MHC II expression, followed by a proliferation of muscle satellite cells. These findings were absent in old mice and in mice deficient in Cx3cr1. Transcriptomic profiling of tissue‐resident skeletal muscle macrophages from old compared with young mice showed downregulation of pathways associated with phagocytosis and proteostasis, and persistent upregulation of inflammatory pathways. Consistently, skeletal muscle macrophages from old mice failed to downregulate MHCII expression during recovery from influenza A virus‐induced pneumonia and showed impaired phagocytic function in vitro. Like old animals, mice deficient in the phagocytic receptor Mertk showed no macrophage expansion, MHCII downregulation, or satellite cell proliferation and failed to recover skeletal muscle function after influenza A pneumonia. Our data suggest that a loss of phagocytic function in a CX3CR1+ tissue‐resident skeletal muscle macrophage population in old mice precludes satellite cell proliferation and recovery of skeletal muscle function after influenza A pneumonia.
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