[HTML][HTML] Tight coupling between glucose and mitochondrial metabolism in clonal β-cells is required for robust insulin secretion
The biochemical mechanisms underlying glucose-stimulated insulin secretion from
pancreatic β-cells are not completely understood. To identify metabolic disturbances in β-
cells that impair glucose-stimulated insulin secretion, we compared two INS-1-derived clonal
β-cell lines, which are glucose-responsive (832/13 cells) or glucose-unresponsive (832/2
cells). To this end, we analyzed a number of parameters in glycolytic and mitochondrial
metabolism, including mRNA expression of genes involved in cellular energy metabolism …
pancreatic β-cells are not completely understood. To identify metabolic disturbances in β-
cells that impair glucose-stimulated insulin secretion, we compared two INS-1-derived clonal
β-cell lines, which are glucose-responsive (832/13 cells) or glucose-unresponsive (832/2
cells). To this end, we analyzed a number of parameters in glycolytic and mitochondrial
metabolism, including mRNA expression of genes involved in cellular energy metabolism …