[HTML][HTML] Direct interaction between endothelial nitric-oxide synthase and dynamin-2: implications for nitric-oxide synthase function
S Cao, J Yao, TJ McCabe, Q Yao, ZS Katusic… - Journal of Biological …, 2001 - ASBMB
Endothelial nitric-oxide synthase (eNOS) is regulated in part through specific protein
interactions. Dynamin-2 is a large GTPase residing within similar membrane compartments
as eNOS. Here we show that dynamin-2 binds directly with eNOS thereby augmenting
eNOS activity. Double label confocal immunofluorescence demonstrates colocalization of
eNOS and dynamin in both Clone 9 cells cotransfected with green fluorescent protein-
dynamin and eNOS, as well as in bovine aortic endothelial cells (BAEC) expressing both …
interactions. Dynamin-2 is a large GTPase residing within similar membrane compartments
as eNOS. Here we show that dynamin-2 binds directly with eNOS thereby augmenting
eNOS activity. Double label confocal immunofluorescence demonstrates colocalization of
eNOS and dynamin in both Clone 9 cells cotransfected with green fluorescent protein-
dynamin and eNOS, as well as in bovine aortic endothelial cells (BAEC) expressing both …