Persistence of dead-cell bacterial DNA in ex vivo root canals and influence of nucleases on DNA decay in vitro
M Brundin, D Figdor, C Roth, JK Davies… - Oral Surgery, Oral …, 2010 - Elsevier
OBJECTIVE: The fate of DNA from bacteria that do not survive in the root canal is uncertain,
yet DNA longevity may confound recovery of authentic etiologic participants in the disease
process. This study assessed the recovery of PCR-detectable DNA in ex vivo human root
canals and some environmental factors on the decay of microbial DNA. STUDY DESIGN:
Heat-killed Enterococcus faecalis cells were inoculated into instrumented human root canals
ex vivo, and samples were taken at intervals over 2 years and analyzed by polymerase …
yet DNA longevity may confound recovery of authentic etiologic participants in the disease
process. This study assessed the recovery of PCR-detectable DNA in ex vivo human root
canals and some environmental factors on the decay of microbial DNA. STUDY DESIGN:
Heat-killed Enterococcus faecalis cells were inoculated into instrumented human root canals
ex vivo, and samples were taken at intervals over 2 years and analyzed by polymerase …