[HTML][HTML] 1, N 2-Ethenoguanine, a Mutagenic DNA Adduct, Is a Primary Substrate of Escherichia coliMismatch-specific Uracil-DNA Glycosylase and Human Alkylpurine …
M Saparbaev, S Langouët, CV Privezentzev… - Journal of Biological …, 2002 - ASBMB
The promutagenic and genotoxic exocyclic DNA adduct 1, N 2-ethenoguanine (1, N 2-εG) is
a major product formed in DNA exposed to lipid peroxidation-derived aldehydes in vitro.
Here, we report that two structurally unrelated proteins, theEscherichia coli mismatch-
specific uracil-DNA glycosylase (MUG) and the human alkylpurine-DNA-N-glycosylase
(ANPG), can release 1, N 2-εG from defined oligonucleotides containing a single modified
base. A comparison of the kinetic constants of the reaction indicates that the MUG protein …
a major product formed in DNA exposed to lipid peroxidation-derived aldehydes in vitro.
Here, we report that two structurally unrelated proteins, theEscherichia coli mismatch-
specific uracil-DNA glycosylase (MUG) and the human alkylpurine-DNA-N-glycosylase
(ANPG), can release 1, N 2-εG from defined oligonucleotides containing a single modified
base. A comparison of the kinetic constants of the reaction indicates that the MUG protein …
