Spatio-temporal transgene regulation by transgenic DNA recombinases is a central tool for
genetic research in multicellular organisms, with excellent applications for misexpression
and lineage tracing experiments. Cre recombinase-controlled lox site recombination is a
cornerstone of contemporary mouse genetics, and Cre/lox techniques therefore attract
increasing interest in the zebrafish field. Tol2-mediated zebrafish transgenesis now provides
a stable platform for lox cassette transgenes, while the ease of drug treatments in zebrafish …