Downregulation of PTEN/MMAC/TEP1 expression in human prostate cancer cell line DU145 by growth stimuli
DR Bastola, GS Pahwa, MF Lin, PW Cheng - Molecular and cellular …, 2002 - Springer
DR Bastola, GS Pahwa, MF Lin, PW Cheng
Molecular and cellular biochemistry, 2002•SpringerGenetic alterations and/or deletion of the tumor suppressor gene PTEN/MMAC/TEP1 occur
in many types of human cancer including prostate cancer. We describe the production of
monoclonal antibody against recombinant human PTEN and the study of PTEN gene and
protein expression in three commercially available human prostate cancer cell lines, PC-3,
LNCaP, and DU 145. Northern blotting analyses showed that LNCaP and DU145 but not PC-
3 cells expressed PTEN mRNA. However, Western blotting analyses using a monoclonal …
in many types of human cancer including prostate cancer. We describe the production of
monoclonal antibody against recombinant human PTEN and the study of PTEN gene and
protein expression in three commercially available human prostate cancer cell lines, PC-3,
LNCaP, and DU 145. Northern blotting analyses showed that LNCaP and DU145 but not PC-
3 cells expressed PTEN mRNA. However, Western blotting analyses using a monoclonal …
Abstract
Genetic alterations and/or deletion of the tumor suppressor gene PTEN/MMAC/TEP1 occur in many types of human cancer including prostate cancer. We describe the production of monoclonal antibody against recombinant human PTEN and the study of PTEN gene and protein expression in three commercially available human prostate cancer cell lines, PC-3, LNCaP, and DU 145. Northern blotting analyses showed that LNCaP and DU145 but not PC-3 cells expressed PTEN mRNA. However, Western blotting analyses using a monoclonal antibody against PTEN demonstrated the expression of PTEN protein in DU145 but not LNCaP cells. In DU145 cells, PTEN expression at both the mRNA and protein levels inversely correlated with serum concentrations and levels of PKB/Akt phosphorylation. In addition, the basal activity of PKB/Akt as indicated by level of phosphorylation was higher in prostate cancer cells which do not express PTEN than that in the cells expressing wild type PTEN. Thus, PTEN may play a critical role in regulating cellular signaling in prostate cancer cells.
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