Sequences of human beta-globin mRNA were determined by analysis of complementary
DNA. beta-mRNA was transcribed into double-stranded cDNA by RNA-dependent DNA
polymerase. cDNA was cut by restriction endonucleases and the fragments were terminally
labeled by means of polynucleotide kinase and [gamma-32P] ATP. After purification,
fragments were degraded by snake venom phosphodiesterase. Alternatively single-
stranded [32P] cDNA was prepared by transcription in the presence of [alpha-32P] dCTP …